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目的观察自制包裹氟碳气体的微泡超声造影剂对微波消融治疗肿瘤的增效作用,并探讨其增效机制。方法首先,制备包裹全氟丙烷(C3F8)气体的脂质微泡。然后做离体、在体研究。实验分为五组:A组,MWA;B组,MWA+MBs;C组,Flash条件下MWA;D组,Flash条件下MWA+MBs;E组,Flash条件下MWA+磷酸盐缓冲液。观察并测量治疗后各组靶区灰度变化面积、灰度值及凝固性坏死范围。结果果比较组间灰度变化面积、灰度值及消融后坏死范围:A组与B组无明显差异(P>0.05),C组大于A、B组(P<0.05),C组与E组无明显差异(P>0.05),D组大于C、E组(P<0.05)。结论包裹氟碳气体的微泡造影剂能增大MWA的治疗范围,此效应可能与Flash条件下爆破微泡产生的空化效应、改变组织声环境、增加微波治疗的热效应有关。
Objective To observe the synergic effect of self-made fluorocarbon gas microbubble ultrasound contrast agent on microwave ablation and to investigate its synergistic mechanism. Methods First, lipid microbubbles encapsulating perfluoropropane (C3F8) gas were prepared. Then do ex vivo, in vivo studies. The experiment was divided into five groups: Group A, MWA; Group B, MWA + MBs; Group C, MWA under Flash; Group D, MWA + MBs under Flash; Group E, MWA + Phosphate buffer under Flash conditions. Observed and measured the target area after treatment, the gray area, gray value and coagulation necrosis range. Results The gray area, gray value and post-ablation necrosis range of group A and group B were no significant difference (P> 0.05), group C was larger than group A and B (P <0.05), group C and E There was no significant difference between the two groups (P> 0.05). The D group was greater than the C and E groups (P <0.05). CONCLUSION: Fluorocarbon gas microbubble contrast agent can increase the therapeutic range of MWA. This effect may be related to the cavitation effect of microbubble bursting under the condition of Flash, changing the tissue acoustic environment and increasing the thermal effect of microwave treatment.