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目的 :研究氯化钐 ( Sm Cl3 )、氯化镨 ( Pr Cl3 )对地塞米松 ( DX)所致免疫功能低下小鼠腹腔巨噬细胞 ( PM)的影响。方法 :选用昆明小鼠连续肌注 DX建立免疫功能低下模型后 ,腹腔注射0 .0 5mg/kg,0 .5mg/kg Sm Cl3 和 Pr Cl3 ,连续 7d,采用定量细胞化学、电子显微镜及吞噬实验方法观察 PM的形态结构及功能。结果 :两种剂量 Sm Cl3 和 Pr Cl3 对 DX所致免疫功能低下小鼠 PM,可使酸性磷酸酶 ( ACP)、α-醋酸萘酚酯酶 ( ANAE)含量和刀豆蛋白 A受体 ( Con AR)数目明显增多。电镜下 ,PM胞质内溶酶体增多 ,表面皱褶及微绒毛增多。吞噬中性红染料的能力增强。结论 :Sm Cl3和 Pr Cl3 对免疫功能低下小鼠 PM的形态结构及功能有明显的刺激作用
Objective: To study the effects of SmCl3 and Pr Cl3 on peritoneal macrophages (PM) in immunocompromised mice induced by dexamethasone (DX). Methods: Kunming mice were intramuscularly injected with DX to establish a model of immunocompromised mice. After intraperitoneal injection of 0 .05 mg / kg, 0.5 mg / kg Sm Cl3 and Pr Cl3 for 7 days, quantitative cytochemistry, electron microscopy and phagocytosis Methods To observe the morphological structure and function of PM. Results: The two doses of SmCl3 and Pr Cl3 could inhibit the activity of ACP, ANAE and Con (Con AR) increased significantly. Electron microscopy, PM cytoplasmic lysosome increased, surface folds and microvilli increased. The ability to devour neutral red dye is enhanced. Conclusion: Sm Cl3 and Pr Cl3 have obvious stimulating effect on the morphological structure and function of PM in immunocompromised mice