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目的强脉冲噪声暴露后,豚鼠耳蜗听神经复合动作电位(Cochlear action potential,CAP)阀值提高,按不同的阈移分为五组:0~5 dB(6只);10~15 dB(5只);20~25 dB(11只);30~35 dB(7只);55~60 dB(5只)以及正常对照组6只豚鼠。方法用Image-pro plus图象分析软件,人机交互对话方式对各组耳蜗铺片观察耳蜗传出神经末梢处病变的长度、传出神经末梢数目以及乙酰胆碱酯酶(AChE)活性(以灰度表示)进行测量。结果不同阈移组间耳蜗病变长度差异均有显著性或极显著性;各实验组耳蜗传出神经末梢计数随阈移增大而减少,与对照组相比差异均有显著性;AChE灰度值变化多集中在第二圈,第二圈传出神经末梢个数与该圈AChE活性呈正相关(r=+0.75);第二圈AChE灰度值变化与CAP阈移相关,即CAP阈移愈大,AChE灰度值愈大(活性降低),反之亦然。结论生理的变化与形态学计量的变化是相一致的。
After exposure to strong impulsive noise, the Cochlear action potential (CAP) threshold of guinea pig cochlear increased, divided into five groups according to different threshold shifts: 0 ~ 5 dB (6), 10 ~ 15 dB ); 20-25 dB (11); 30-35 dB (7); 55-60 dB (5) and 6 guinea pigs in the normal control group. Methods Image-pro plus image analysis software and human-computer interactive dialogue method were used to observe the length of the cochlear exocrine nerve terminals, the number of nerve terminals and the activity of acetylcholinesterase (AChE) ). Results There were significant or significant differences in the length of cochlear lesions between different threshold shift groups. The count of cochlear exocentric nerve terminals in each experimental group decreased with the increase of threshold shift, the difference was significant compared with the control group. The AChE gray scale The changes of AChE value in the second circle were correlated with the threshold of CAP shift, that is, the threshold of CAP shift (r = +0.75) The larger the AChE grayscale value (less active) and vice versa. Conclusions Physiological changes are consistent with morphometric changes.