冬小麦花药培养受到诸多内外条件的制约,培养温度是其中最重要的外界条件之一.已有不少报道表明培养温度明显影响小麦花药培养的效果.本文仅报道本室近几年在这方面的一些试验结果.材料与方法供试材料为冬小麦的一些品种和一些F_1杂种.取单核中后期的花药,用常规无菌操作法接种到改良N_6培养基上.经过愈伤组织分化出绿苗,去分化阶段在黑暗中培 The anther culture of winter wheat is restricted by many internal and external conditions, and the culture temperature is one of the most important external conditions.Numerous reports have shown that the culture temperature significantly affects the anther culture of wheat.In this paper, Some of the experimental results.Materials and Methods The test materials were some varieties of winter wheat and some F_1 hybrids.The anthers of the middle and late monomembranes were inoculated into the modified N_6 medium by the conventional aseptic method.They differentiated green calluses , To the differentiation stage in the dark training