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目的探讨检测胃癌腹膜转移诊断的方法。方法收集50例胃癌患者(胃癌组)及10例胃良性病变患者的腹腔冲洗液,并以胃癌原发灶组织标本作为对照。采用流式细胞术检测标本中肿瘤标志物CEA,采用HE染色进行腹腔冲洗液细胞学(PLC)检查,同时分析上述资料的临床意义。结果胃癌组腹腔冲洗液中CEA阳性表达率为54.0%(27例),明显高于PLC所检测的24.0%(12例)的阳性率(P<0.05)。阳性检出率随着肿瘤浸润深度,TNM分期,胃壁受侵程度的增加而增加。胃癌组原发灶中CEA的阳性表达率为86.0%(43例),对照组腹腔冲洗液中CEA无阳性表达。结论流式细胞术检测腹腔冲洗液中CEA可作为预测胃癌腹膜种植转移的手段。
Objective To investigate the method of detecting peritoneal metastasis of gastric cancer. Methods 50 cases of gastric cancer patients (gastric cancer group) and 10 cases of gastric benign lesions in patients with peritoneal wash fluid, and the primary gastric cancer tissue specimens as a control. The tumor marker CEA was detected by flow cytometry and the peritoneal wash cytology (PLC) was performed by HE staining. Meanwhile, the clinical significance of the above data was analyzed. Results The positive rate of CEA in gastric cancer group was 54.0% (27 cases), which was significantly higher than that of PLC (24.0%, 12 cases) (P <0.05). Positive detection rate with the depth of tumor invasion, TNM staging, gastric wall invasion increased. The positive expression rate of CEA in gastric cancer group was 86.0% (43 cases), but there was no positive CEA in the control group. Conclusion Flow cytometry in the peritoneal wash fluid CEA can be used as a means of predicting peritoneal metastasis of gastric cancer.