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为探索利用高效液相色谱法测定设施番茄土壤低分子量有机酸的适宜色谱条件,以Na OH和Na Cl混合溶液为提取剂提取11种土壤低分子量有机酸,通过优化影响低分子量有机酸分离度的流动相浓度、p H、流速、有机改良剂的添加比例、检测波长等条件,确定了高效液相色谱法测定设施番茄土壤低分子量有机酸的最佳色谱条件为:以10 mmol L-1KH_2PO_4-CH_3OH(97∶3,p H 2.6)为流动相,流速为0.8 ml min~(-1),检测波长220 nm,柱温25℃,进样量为20μl。此方法对11种低分子量有机酸的检测限在20~825 ng ml~(-1)之间,回收率在85.57%~100.20%之间,相对标准偏差在0.17%~5.84%之间。实际土壤样品分析结果表明,该方法能够满足土壤低分子量有机酸的痕量检测要求。
In order to explore the suitable chromatographic conditions for the determination of low molecular weight organic acids in tomato soils using high performance liquid chromatography (HPLC), eleven kinds of organic acids with low molecular weight were extracted by using NaOH and NaCl as extracting agents. By optimizing the separation of low molecular weight organic acids , The mobile phase concentration, p H, flow rate, addition ratio of organic modifier and detection wavelength, the optimum chromatographic conditions for the determination of low molecular weight organic acids in tomato soil by high performance liquid chromatography were determined as follows: 10 mmol L -1 KH 2 PO 4 The mobile phase was CH_3OH (97: 3, p H 2.6) with a flow rate of 0.8 ml min -1. The detection wavelength was set at 220 nm and the column temperature was 25 ℃. The injection volume was 20 μl. The detection limit of 11 low molecular weight organic acids was between 20 and 825 ng ml -1. The recoveries ranged from 85.57% to 100.20%. The relative standard deviations ranged from 0.17% to 5.84%. The actual soil sample analysis results show that this method can meet the trace detection requirements of low molecular weight organic acids in soil.