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随着薄层色谱扫描仪的开发和应用,薄层扫描法(TLCS)在色谱分析中愈来愈占有重要的地位。在药物分离,鉴定,定量分析中发挥着重要的作用。根据薄层扫描的检测原理,可把TLCS分为两大类,即薄层紫外扫描法(TLCUS)和薄层荧光扫描法(TLCFS),其中TLCUS已应用的广泛,日臻完善,而TLCFS的应用目前尚不够广泛,然而,TLCFS具有灵敏度高,选择性好的优点,对普通薄层板的检出灵敏度为0.1～1ng,对高效薄层板的检出灵敏度为0.01～0.1ng,比TLCUS的灵敏度高10~1～10~3倍。本文试图对这种
With the development and application of thin-layer chromatography scanners, thin-layer chromatography (TLCS) plays an increasingly important role in chromatographic analysis. It plays an important role in drug separation, identification, and quantitative analysis. According to the detection principle of thin-layer scanning, TLCS can be divided into two major categories, namely Thin-Layer Ultraviolet Scanning (TLCUS) and Thin-Layer Fluorescence Scanning (TLCFS). Among them, TLCUS has been widely used and is being improved, while the application of TLCFS has been improved. At present, it is not yet widely available. However, TLCFS has the advantages of high sensitivity and good selectivity. The detection sensitivity for ordinary thin-layer plates is 0.1 to 1 ng, and the detection sensitivity for high-performance thin-layer plates is 0.01 to 0.1 ng, which is better than that of TLCUS. The sensitivity is 10~1~10~3 times higher. This article tries to do this