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目的方法利用ＺｈｏｕＪｉａｎ教授提供的重组质粒ＤＮＡｐＢＶ２２０／ｅＡｇ，转染ＤＨ５α细胞，经３０℃培养３小时，４２℃培养６小时温度诱导后，提取乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）。经ＤＥＡＥ－ＳｅｐｈａｒｏｓｅＦａｓｔＦｌｏｗ层折纯化后免疫打点分析，收集阳性蛋白。经ＳＤＳ－ＰＡＧＥ电泳，考马斯亮蓝Ｇ２５０染色，显示为单一的蛋白带，用免疫印迹法（Ｗｅｓｔｅｒｎｂｌｏｔ）和免疫打点（ｌｍｍｕｎｏｄｏｔ）法，确定表达产物的特异性。将纯化的ＨＧｅＡｇ用酶联免疫吸附试验（ＥＬＩＳＡ）和免疫条。方法基检测人血清中的相关抗体。结果经４９例乙型肝炎病毒ｅ抗体（ＨＢｅＡｂ）阳性病人血清检验证实了其特异性。结论免疫条方法较ＥＬＩＳＡ方法更敏感、特异。
Objectives The method uses the recombinant plasmid DNApBV220 / eAg provided by Professor ZhouJian to transfect DH5α cells, which is cultured for 3 hours at 30 ° C. and is induced at a temperature of 42 ° C. for 6 hours to extract the hepatitis B virus e antigen (HBeAg). After DEAE-SepharoseFastFlow layer purification purification immune RBI analysis, collecting positive protein. After SDS-PAGE electrophoresis, Coomassie brilliant blue G250 staining showed a single protein band. The specificity of the expressed product was determined by Western blot and lmmunodot method. Purified HGeAg was subjected to enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Method based detection of human serum related antibodies. The results of 49 cases of hepatitis B virus e antibody (HBeAb) -positive patients serum confirmed its specificity. Conclusion Immuno-strip method is more sensitive and specific than ELISA method.