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本研究探讨干扰素α(IFN-α)对慢性髓系白血病(CML)来源树突状细胞(DC)表达趋化因子CCR7及分泌IL-10、IL-12P70的影响。在诱导CML-DCs的无血清条件培养基中,除加入SCF、GM-CSF、TNF-α及IL-4外,还加入不同浓度IFN-α。培养10-14天后,用流式细胞术检测共刺激分子及CCR7表达,G显带法显示Ph1染色体,噻唑蓝(MTT)法检测CML-DC刺激正常人外周血淋巴细胞增殖状况,ELISA法检测培养上清IL-10及IL-12P70含量。结果显示:IFN-α(300U/ml)组与无IFN-α组比较其CML-DC的CD40、CD83、CD86及CCR7的表达均升高1倍,异体混合淋巴细胞反应(MLR)中OD值增加1倍,Ph1染色体阳性比例及IL-10和IL-12P70浓度均减低。结论:IFN-α能够部分纠正CML-DC免疫表型及功能缺陷,这同其上调DC共刺激分子和CCR7表达及解除了CML血清中IL-10对CML-DC分化的抑制有关。
This study was designed to investigate the effects of IFN-α on the expression of chemokine CCR7 and IL-10 and IL-12P70 induced by dendritic cells from chronic myeloid leukemia (CML). In the serum-free conditioned medium of induced CML-DCs, different concentrations of IFN-α were added in addition to SCF, GM-CSF, TNF-α and IL-4. After culturing for 10-14 days, the expression of costimulatory molecules and CCR7 were detected by flow cytometry. Ph1 chromosome was detected by G banding method. Proliferation of peripheral blood lymphocytes stimulated by CML-DC was detected by MTT assay. Culture supernatant IL-10 and IL-12P70 content. The results showed that the expression of CD40, CD83, CD86 and CCR7 in CML-DCs in IFN-α (300U / ml) group was doubled compared with that in IFN-α group. OD value in allogeneic mixed lymphocyte reaction A 1-fold increase, Ph1 chromosome positive ratio and IL-10 and IL-12P70 concentrations were reduced. CONCLUSION: IFN-α can partly correct the immunophenotypic and functional defects of CML-DC, which is related to its upregulation of DC costimulatory molecules and CCR7 expression and the release of CML-DC from CML serum.