论文部分内容阅读
目的探讨FQ-PCR技术对活动性骨关节结核和泌尿系结核的诊断价值。方法选择118例疑似结核病患者,其中关节炎患者52例,泌尿系感染患者66例,应用FQ-PCR技术分别检测患者关节腔积液和尿液中的TB-DNA,并与抗酸染色法的检出率比较。结果 52例关节炎患者中,确诊结核性关节炎患者36例,TB-DNA阳性22例,22例全部为确诊病例,阳性率61.1%。抗酸染色的阳性数为2,均为TB-DNA阳性者,阳性率5.6%;66例泌尿系统疾病患者中,确诊泌尿系结核患者52例,TB-DNA阳性32例,32例全部为确诊病例,阳性率为61.5%,抗酸染色的阳性数为4,均为TB-DNA阳性者,阳性率7.7%。结论应用FQ-PCR技术检测患者关节腔积液和尿液中的TB-DNA,具有高度敏感性和特异性,可作为结核性关节炎和泌尿系统结核早期诊断的一个可靠指标。
Objective To investigate the diagnostic value of FQ-PCR in active bone and joint tuberculosis and urinary tuberculosis. Methods One hundred and eighteen patients with suspected TB were selected, including 52 cases of arthritis and 66 cases of urinary tract infection. The TB-DNA in joint effusion and urine was detected by FQ-PCR, Detection rate comparison. Results Of the 52 patients with arthritis, 36 cases were diagnosed as tuberculous arthritis, 22 cases were positive for TB-DNA, and 22 cases were all confirmed cases, the positive rate was 61.1%. The positive number of acid-fast staining was 2, all positive for TB-DNA, the positive rate was 5.6%. Among the 66 cases of urinary system diseases, 52 cases of urinary tuberculosis were diagnosed, TB-DNA positive was 32 cases, 32 cases were diagnosed The positive rate was 61.5% and the positive number of acid-fast staining was 4, both positive for TB-DNA, the positive rate was 7.7%. Conclusion The detection of TB-DNA in joint effusions and urine by FQ-PCR is highly sensitive and specific and can be used as a reliable indicator of early diagnosis of tuberculous arthritis and urinary tract tuberculosis.