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建立灵敏、可靠的大鼠内耳膜迷路线粒体ＤＮＡ（ｍｔＤＮＡ）提取和检测方法。方法：结合ＰＣＲ技术扩增ｍｔＤＮＡ编码ＮＤ１－１６ ＳｒＲＮＡ基因的 ６０１ ｂｐ片段，检测大鼠听泡内耳膜迷路线粒体ＤＮＡ方法，并对两种ｍｔＤＮＡ获取方法进行比较。结果：采用Ｓｅｉｄｍａｎ的方法，将１只大鼠的双侧听泡内耳膜迷路作为一个样品，检测１０个样品均成功扩增出编码ＮＤ １－１６ ＳｒＲＮＡ基因的 ６０１ ｈｐ片段；而采用 Ｅｄｒｉｓ ｍｔＤＮＡ提取方法，需用 ６侧听泡内耳膜迷路作为一个样品方可获得可靠的阳性结果。结论：采用Ｓｅｉｄｍａｎ方法提取内耳膜迷路线粒体ＤＮＡ，结合ＰＣＲ技术扩增ｍｔＤＮＡ高度保守基因片段，作为总ＤＮＡ中含有被ＰＣＲ扩增量ｍｔＤＮＡ存在指标，可应用于内耳膜迷路ｍｔＤＮＡ突变的研究。
To establish a sensitive and reliable method for the extraction and detection of mtDNA in rat eardrum. Methods: A 601 bp fragment of mtDNA encoding ND1-16 SrRNA gene was amplified by polymerase chain reaction (PCR), and mitochondrial DNA was detected in the eardrum of the rat with auditory eyelid obstruction. The two mtDNA acquisition methods were compared. RESULTS: Seidman’s method was used to detect the labyrinth of the inner ear membrane of one rat as a sample. The 601 hp fragment encoding ND 1-16 SrRNA gene was successfully amplified in all 10 samples. The Edris mtDNA was extracted Method, the need to use 6 side to listen to bubble inner ear labyrinth as a sample before obtaining reliable positive results. Conclusion: Mitochondrial DNA was extracted by Seidman method and the mtDNA highly conserved gene fragment was amplified by PCR. As a marker of mtDNA content in total DNA, PCR-amplified mtDNA could be used to detect mtDNA mutations in the inner ear.