In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of restrietion endonuclease physical map is analyzed, the complete genomic library is organized. On basis of this, the eomplete genome nueleotide sequences (32 838 bp in length, including terminal structures) are determined. The data analysis shows: compared with the other Adenoviruses, strain AAV-2 has more disparity on ganomic structure and the distribution of open reading frame (ORF). There are no elear E1, E3 and F4 regions in AAV-2 genome. Two segments located at both ends of genome (1.1 kb and 8.3 kb in length respectively) have no homology with the other adenovirus genomes. In addition, strain AAV-2 genome lacks ORFs encoding E1A, pⅤ and pⅨ, which are common ORFs encoding early, lately proteins in Adenovirus. This reveals differences between EDSA-76, the sole standard strain of group Ⅲ Avian Adenoviruses, and the other Avian Adenoviruses for the first time. It will help the search for Avian Adenovirus In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of resumption endonuclease physical map is analyzed, the complete genomic library is organized. On basis of this, the eomplete genome nueleotide sequences (32 838 bp in length, including terminal structures) are determined. The data analysis shows: compared with the other Adenoviruses, strain AAV-2 has more disparity on ganomic structure and the distribution of open reading frame (ORF). There are no elear E1, E3 and F4 regions in AAV-2 genome. Two segments located at both ends of genome (1.1 kb and 8.3 kb in respectively respectively) have no homology with the other adenovirus genomes. In addition, strain AAV-2 genome lacks ORFs encoding E1A, pV and pIX, which are common ORFs encoding early, lately proteins in Adenovirus. This reveals differences between between EDSA-76, the sole standard strain of group III Avian Adenoviruses, and the other Avian Adenoviruses for the first time. It will help the search for Avian Adenovirus