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741杨是用有性杂交法选育出的一个优良毛白杨杂种无性系。用组培法经正交试验,获得了春季芽继代培养基成份的最佳组合:1/2G.MS+2.5%+6-BA0.5+KT0.1+IBA0.03,分化率为85%。芽继代培养基加入植物生长调节剂的浓度应随季节变化而改变。对生根培养基的研究发现,生根基质用琼脂比蛭石效果好。并提出了一套较成熟的试管苗移栽到大田的技术措施。
Poplar 741 is a clone of Populus tomentosa hybrids bred by sexual hybridization. Orthogonal test by tissue culture method, the best combination of spring bud subculture medium was obtained: 1 / 2G.MS + 2.5% + 6-BA0.5 + KT0.1 + IBA0.03, the differentiation rate was 85 %. The concentration of shoot subculture medium added to the plant growth regulator should change with the season. The study on rooting medium found that rooting substrate agar was better than vermiculite. And put forward a set of more mature test-tube plantlets transplanted to the field of technical measures.