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目的:观察缺血预处理对在体兔肺常温缺血再灌注损伤脂质过氧化的影响。方法:采用阻断左肺门的肺缺血再灌注损伤模型。硫代巴比妥酸法及UFN13直接法测肺组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷航甘肽过氧化物酶(GSHpx)及肺组织光镜观察。结果:缺血再灌组左肺MDA较假手术组和缺血组为高,而SOD和GSHpx活性较低(P<0.01),肺组织损伤较重。与缺血再灌组比较,缺血预处理+缺血再灌组有较低的MDA含量和较高的SOD与GSHpx活性(P<0.05),肺损伤亦较轻。结论:缺血预处理可减轻兔肺常温缺血再灌注诱导的脂质过氧化反应。
Objective: To observe the effect of ischemic preconditioning on lipid peroxidation induced by ischemia-reperfusion in the lungs of rabbits at normal temperature. Methods: The model of lung ischemia-reperfusion injury with left hilar was occluded. Thiobarbituric acid method and UFN13 direct method were used to detect the contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSHpx) and lung tissue in lung tissue. Results: The left lung MDA in ischemic reperfusion group was higher than that in sham operation group and ischemia group, while SOD and GSHpx activities were lower (P <0.01). The lung injury was heavier. Compared with ischemia / reperfusion group, ischemia preconditioning + ischemia / reperfusion group had lower MDA content and higher activity of SOD and GSHpx (P <0.05), and lung injury was also lighter. Conclusion: Ischemic preconditioning can attenuate the lipid peroxidation induced by ischemia reperfusion in rabbits lung.