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以苹果属砧木种对缺铁敏感的山定子(Malus baccata(L.)Borkh.)和对缺铁耐受的西府海棠(Malus micromalus Makino)为材料,用无菌苗茎段建立了可继代培养的愈伤组织试验体系。比较了两种愈伤组织在不同铁营养条件下其生理与生化的变化。山定子愈伤组织在铁逆境下,其干重和鲜重显著增加,P,S,Mg,K,Zn,B等元素含量也均有所增加,三价铁还原能力未发生变化,细胞的超微结构遭到一定损伤,并在加重碳酸根离子(HCO_3~-)的缺铁逆境下合成了一种25kD的多肽。相反,西府海棠愈伤组织在缺铁条件下,干重和鲜重未发生显著变化,P和S元素含量显著降低,三价铁还原能力增加,并形成了传递细胞,在低铁和低铁加重碳酸根离子逆境下,合成了25kD,34kD和100kD的多肽。以上结果表明,对缺铁敏感的山定子和对缺铁耐受的西府海棠对缺铁营养逆境的反应不同,这是由其不同的遗传基础决定的。本结果为今后培育耐缺铁新类型提供了理论依据。
In this study, Malus baccata (L.) Borkh., An apple rootstock species sensitive to iron deficiency, and Malus micromalus Makino, an iron-tolerant species, Cultured callus test system. The physiological and biochemical changes of two kinds of callus under different iron nutrition conditions were compared. Under the iron stress, the dry weight and fresh weight of the mountain stator callus increased significantly, the content of P, S, Mg, K, Zn, B and other elements also increased, while the ferric reduced capacity did not change. The ultrastructure was damaged to a certain extent, and a 25kD polypeptide was synthesized under the stress of iron deficiency which increased the concentration of carbonate ions (HCO 3 -). On the contrary, under the conditions of iron deficiency, no significant changes were observed in the dry weight and fresh weight of the West Begonia callus while P and S contents were significantly reduced and the ferric reduced capacity was increased and the cells were transferred. In the low iron and low iron Under the stress of carbonate ion, peptides of 25kD, 34kD and 100kD were synthesized. The above results indicate that the responsiveness to iron deficiencies of mountain statins susceptible to iron deficiency and iron tolerance to iron deficiency of Begonia is determined by their different genetic basis. The results provide a theoretical basis for the future cultivation of new types of iron deficiency resistance.