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采用基因芯片技术,以超级杂交稻先锋组合两优培九及其亲本和分离世代F2代的优势集团和负优势集团为材料,尝试利用水稻表达谱芯片分析水稻杂种优势的分子机理。结果表明,以杂种F1(两优培九)为对照组,亲本9311、培矮64S以及F2代的优势集团、负优势集团的有效基因数分别为8304,8962,9956,10196个,差异表达基因数分别有447,122,617,388个,这些差异表达基因涉及代谢、发育、运输和蛋白修饰等多种功能,但其中大部分基因的功能尚未知。提出了差异表达基因的显性表达水平的概念。分析表明,具显性表达水平的差异表达基因的聚合与杂种优势表现无明显相关性。
Using gene chip technology, the molecular mechanism of rice heterosis was analyzed by rice gene expression profiling using the superior hybrid rice combination Liangyou Peijiu and its parents and the F2 generations of the dominant group and the negative dominant group of super hybrid rice as materials. The results showed that there were 8304, 8962, 9956 and 10196 dominant genes in the dominant group and negative dominant group of 9311, Pei’ai 64S and F2 generation, respectively, with the hybrid F1 (Liangyoupeijiu) as the control group. The number of differentially expressed genes 447,122,617,388, these differentially expressed genes involved in metabolism, development, transport and protein modification and many other functions, but most of the gene’s function is unknown. The concept of the dominant expression level of differentially expressed genes was proposed. Analysis showed that there was no significant correlation between the polymerization of differentially expressed genes with dominant expression level and heterosis.