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乙肝病毒(HBV)逆转录酶(RT)和HBV S抗原(HBVsAg)基因间的重叠如何调节HBV基因变化的程度仍是一个令人不解的问题,本文对此进行了研究。由于RT和HBsAg之间的重叠,RT基因的非典型模式提高了核苷酸的保守率(变异性≤1%)(重叠区的核苷酸保守率为69.9%,而非重叠区的则为41.2%;P<0.001),其结果是在重叠区内的同义替代率较低[中间数dS=3.1(1.5~7.4)对20.1(10.6~30.0);P=3.249×10-22]。最保守的RT区位于RT功能性关键的掌区与指区的YMDD模体内
The extent to which the overlap of the HBV reverse transcriptase (RT) and HBV S antigen genes (HBVsAg) regulate the degree of HBV genetic alterations remains a puzzling issue and is examined in this paper. Due to the overlap between RT and HBsAg, the atypical pattern of the RT gene increased the nucleotide conservation (variability <1%) (nucleotide overlap in the overlap region was 69.9%, whereas the non-overlap region was 41.2%; P <0.001). The result was a lower rate of synonymous substitution in the overlap region (median dS = 3.1 (1.5-7.4) vs 20.1 (10.6-30.0); P = 3.249x10-22]. The most conserved RT region is located within the YMDD motif of the RT-functional palms and finger regions