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目的 :在已建成庚型肝炎病毒 (HGV)转基因小鼠模型的基础上 ,研究 HGV包膜蛋白 E2在转基因小鼠体内的表达及定位 ,并探讨 HGV在转基因小鼠体内是否引起病理学改变。 方法 :取小鼠的器官组织用免疫组织化学方法 ,以 HGV E2蛋白的单克隆抗体对 HGV包膜蛋白 E2的表达进行分析和定位。通过血清 AL T检测及常规病理学技术观察转基因小鼠不同器官的病理学变化。 结果 :免疫组织化学结果表明 ,HGV E2主要表达于转基因小鼠肝细胞膜 ,少量表达于胞质内 ;肾脏、心脏、肺及脾脏均未呈现 E2蛋白的阳性反应。组织病理学检查显示转基因小鼠的肝细胞出现水样变性、脂肪变性及淋巴细胞浸润等轻度炎性反应 ,这些变化不随年龄增长而加重 ;其他组织的病理学检查无异常。转基因小鼠血清转氨酶 AL T与对照小鼠无明显差异。 结论 :HGV包膜蛋白 E2的表达具有相对的嗜肝性 ,主要分布在转基因小鼠的肝细胞膜 ,并可引起轻度肝细胞损伤。
OBJECTIVE: To study the expression and localization of HGV envelope protein E2 in transgenic mice based on the established model of hepatitis G virus (GG) transgenic mice and to investigate whether HGV causes pathological changes in transgenic mice. Methods: HGV E2 protein was analyzed by immunohistochemistry and the HGV E2 protein was detected by the monoclonal antibody of HGV E2 protein. Pathological changes in different organs of transgenic mice were observed by serum ALT and routine pathology. Results: The results of immunohistochemistry showed that HGV E2 was mainly expressed in the liver cell membrane of transgenic mice and a small amount in the cytoplasm. No positive reaction of E2 protein was found in kidney, heart, lung and spleen. Histopathological examination revealed mild inflammatory reactions such as watery degeneration, steatosis and lymphocytic infiltration in the liver cells of transgenic mice. The changes did not aggravate with age. The histopathological examination of other tissues showed no abnormality. Transgenic mice serum ALT and control mice no significant difference. CONCLUSION: The expression of HGV envelope protein E2 is relatively hepatophilic, mainly distributed in the liver cell membrane of transgenic mice and can cause mild hepatocellular injury.