Objective To evaluate levels of the circulating antisperm antibody (ASA) in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their outcomes of previous pregnancy, these women were divided into two groups: group HC (having at least one child, n=45), group NC (no child but one to three abortions, n=35). The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. Results Of the 83 patients assessed for ASA-IgG, 14 had immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between 10% and 20%. No case had the clinical positive level according to the WHO criteria. No differences were found on ASA-IgG and ASA-IgA between HC and NC groups (P>0.05). Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility. Objective To evaluate levels of the circulating antisperm antibody (ASA) in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their results of previous pregnancy, these women were divided into two groups: group HC (having at least one child, n = 45), group NC (no child but one to three abortions, n = 35). The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual Results Of the 83 patients assessed for ASA-IgG, 14 had had an immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between No differences were found on ASA-IgG and ASA-IgA between HC and NC groups (P> 0.05). Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility