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建立了液相色谱-串联质谱法同时测定补肾固齿丸中的11种成分。采用C18色谱柱,以甲醇-水为流动相,梯度洗脱。采用多反应离子检测,结合正负离子扫描切换,甜菜碱、芒柄花素、五味子醇甲和23-乙酰泽泻醇B采用正离子方式检测,梓醇、丹参素钠、丹酚酸B、肉桂酸、蒙花苷、柚皮素和丹皮酚采用负离子方式检测。所有被测物的线性关系良好;方法回收率为98.5%～107.0%,RSD为0.7%～3.1%。
A liquid chromatography-tandem mass spectrometry (LC-MS / MS) method was established for simultaneous determination of eleven components in Bushen Guotai Pills. C18 column with methanol - water as mobile phase, gradient elution. Using multi-reactive ion detection, combined with positive and negative ion scan switch, betaine, formononetin, schisandrin and 23-acetyl alisol B detected by positive ion, catalpol, sodium danshensu, salvianolic acid B, Acid, glycosides, naringenin and paeonol negative ion detection. The linearity of all tested materials was good. The recoveries were 98.5% ~ 107.0% with RSD 0.7% ~ 3.1%.