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本工作采用肝部分切除(PH)后96h 大鼠的离体肝细胞,以细胞存活率、介质中 GPT、GOT 含量作为肝损伤指标,观察其抗四氯化碳损伤作用。在不加四氯化碳条件下,肝细胞温育3h 或5h 后,细胞存活率、漏入介质中GPT、GOT 活性在假手术组和 PH 组间无显著差异。但在有四氯化碳损伤条件下则不同,在温育3h 或5h 后,存活率在假手术组分别降到52.8±5.9%和43.5±5.8%,而 PH 组分别为69.2±5.8%和60.9±3.2%。漏出的 GPT和 GOT 活性在假手术组也明显高于 PH 组(P<0.01)。提示大鼠离体再生肝细胞有明显的抗四氯化碳损伤作用。
In this study, liver cells were isolated from rats 96 hours after partial hepatectomy (PH). Cell viability, GPT and GOT contents in the medium were used as indicators of liver injury, and their anti-carbon tetrachloride damage was observed. In the absence of carbon tetrachloride, there was no significant difference in cell viability, leakage of GPT, GOT activity between the sham group and the PH group after incubation of hepatocytes for 3 or 5 hours. However, in the presence of carbon tetrachloride injury, the survival rate decreased to 52.8±5.9% and 43.5±5.8% in the sham group after 3 h or 5 h of incubation, whereas the PH group was 69.2±5.8% and 60.9±3.2%. The leakage of GPT and GOT activity was also significantly higher in the sham group than in the PH group (P<0.01). It suggests that rat isolated regenerative hepatocytes have obvious anti-carbon tetrachloride injury.