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目的:观察骨形成蛋白(BMP-2)、碱性成纤维细胞生长因子(bFGF)和地塞米松(Dex)联合应用对犬牙周膜细胞(PDLCs)成骨分化能力的影响。方法:将第4代犬PDLCs分为5组:bFGF+Dex、BMP-2+bFGF、BMP-2+Dex、BMP-2+bFGF+Dex、对照组。检测各组碱性磷酸酶(ALP)活性,并应用RT-PCR检测各组PDLCs成骨关键基因OPN、COL-1的表达。结果:第7、14 d,各组ALP表达均较空白组显著增强(P<0.05),其中200μg/L BMP-2+10μg/LbFGF+10-8mol/L Dex诱导组犬PDLCs的ALP活性显著高于其他各组(P<0.05)。RT-PCR显示,200μg/L BMP-2+10μg/L bFGF+10-8mol/L Dex诱导组OPN、COL-1基因表达最为显著。结论:3种诱导因子两两结合均能增强犬PDLCs成骨能力,但在最佳浓度下3种因子联合应用诱导能力最强(P<0.05)。
OBJECTIVE: To observe the effects of BMP-2, bFGF and Dex on osteogenic differentiation of periodontal ligament cells (PDLCs). Methods: The 4th generation canine PDLCs were divided into 5 groups: bFGF + Dex, BMP-2 + bFGF, BMP-2 + Dex, BMP-2 + bFGF + Dex. Alkaline phosphatase (ALP) activity of each group was detected. RT-PCR was used to detect the expression of osteoblast OPN and COL-1 in each group. Results: At the 7th and 14th day, the expression of ALP in each group was significantly higher than that in the blank group (P <0.05). The ALP activity of PDLCs in 200μg / L BMP-2 + 10μg / LbFGF + 10-8mol / L Dex group was significant Higher than other groups (P <0.05). RT-PCR showed that the expression of OPN and COL-1 gene in 200μg / L BMP-2 + 10μg / L bFGF + 10-8mol / L Dex group was the most significant. CONCLUSION: The combination of three kinds of inducing factors can enhance the osteogenic capacity of PDLCs. However, the combination of three factors at the optimum concentration has the strongest inducing ability (P <0.05).