我们用ＰＣＲ－ＲＦＬＰ法研究ＤＬＡⅡ类基因。为寻找合适的引物，用四对不同的引物：ＤＬＡ－ＤＲ－ＳＰ／Ｓｔｏｐ，ＤＬＡ－ＤＲ－ＳＰ／Ｐ３，ＨＬＡ－ＤＲＢ－ＧＨ４６／５０及ＨＤＡ－ＤＲＢ－ＡＭＰ－Ａ／Ｂ进行了一系列扩增。只有引物对ＨＬＡ－ＤＲＢ－ＡＭＰ－Ａ／Ｂ获得了满意的结果。其类似核苷酸序列在ＤＬＡ－ＤＲＢｃＤＮＡ的核苷酸序列内被发现，特异性也为Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交分析所证实。内切酶ＨａｅⅢ和ＨｉｎｆⅠ酶解后显示出ＤＬＡ－ＤⅡ类基因高度的多态性和等位基因特异的多态性带型。揭示该引物对是目前用ＰＣＲ－ＲＦＬＰ法研究ＤＬＡ－ＤＲＢ１基因较理想的、实用的引物。 We studied DLA class II genes by PCR-RFLP. In order to find a suitable primer, a series of four different primers were used: DLA-DR-SP / Stop, DLA-DR-SP / P3, HLA-DRB-GH46 / 50 and HDA-DRB-AMP-A / B Amplify. Only the primers obtained satisfactory results for HLA-DRB-AMP-A / B. Similar nucleotide sequences were found within the nucleotide sequence of DLA-DRB cDNA and the specificity was also confirmed by Southern blot hybridization analysis. After digestion with Hae III and Hinf I, the polymorphisms of DLA-DⅡgene and allele-specific polymorphism bands were revealed. This primer pair is the ideal and practical primer for the study of DLA-DRB1 gene by PCR-RFLP.