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人类免疫缺陷病毒Ⅰ型 (humanimmunodeficiencyvirustypeⅠ ,HIV Ⅰ )为慢病毒家族成员之一 ,除 gag ,pol与env等结构基因外 ,其结构中还含有编码 2个调节蛋白及 4个附属蛋白的基因。目前已构建了多种类型的复制缺陷性HIV Ⅰ载体 ,产生的病毒滴度最高可达 10 7TU /ml;对包装细胞系的研究发现 ,将 4个附属基因全部去除对载体的转导能力并无显著影响。基因组分离式包装系统降低了产生具有复制能力的病毒的可能性。HIV Ⅰ载体具有两大特点 :可有效转导人CD34+造血干 /祖细胞、重复注射不引起排斥反应。改建后的HIV Ⅰ载体具有广泛的宿主范围。
Human immunodeficiency virus type Ⅰ (HIV Ⅰ) is a member of the lentivirus family. In addition to the structural genes such as gag, pol and env, its structure contains genes encoding two regulatory proteins and four accessory proteins. At present, many types of replication-deficient HIV-1 vectors have been constructed and the highest titer of virus produced is 10 7 TU / ml. Studies on packaging cell lines have found that all four accessory genes are transduced with vector No significant effect. The genome-separated packaging system reduces the possibility of producing replication-competent viruses. HIV I vector has two major characteristics: can effectively transduce human CD34 + hematopoietic stem / progenitor cells, repeated injection does not cause rejection. The reformed HIV-1 vector has a wide host range.