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目的研究PBK/TOPK在HU(羟基脲)诱导白血病细胞K562的分化时的表达及意义。方法用不同浓度的羟基脲对K562细胞进行诱导分化实验,通过联苯胺、吉姆萨-瑞氏染色和流式细胞仪证实其分化方向;用Western blot法检测PBK/TOPK在K562细胞分化前后表达的变化。结果400μmol/L的羟基脲对K562作用4d后具有良好的诱导分化效果,并诱导其向红系分化,且PBK/TOPK在K562细胞向成熟分化后表达不变,而Pho-PBK/TOPK和Pho-p38有少量增加。结论HU能抑制白血病细胞K562的增殖,并诱导其向红系分化,在此过程中,有激活的PBK/TOPK参与,推测很可能通过磷酸化下游分子p38而起作用。
Objective To study the expression and significance of PBK / TOPK in the differentiation of K562 cells induced by HU (hydroxyurea). Methods K562 cells were induced to differentiate with different concentrations of hydroxyurea. The differentiation of K562 cells was confirmed by benzidine, Giemsa-Wright staining and flow cytometry. The expression of PBK / TOPK was detected by Western blot before and after K562 cells were differentiated Variety. Results After treated with 400μmol / L hydroxyurea for 4 days, K562 cells had good differentiation and induced erythroid differentiation, and PBK / TOPK remained unchanged in K562 cells after maturation. Pho-PBK / TOPK and Pho -p38 There is a small increase. Conclusions HU can inhibit the proliferation of K562 leukemia cells and induce erythroid differentiation. In this process, activated PBK / TOPK are involved, suggesting that HU may play a role in phosphorylation of downstream molecule p38.