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目的探讨职业钒接触和热休克蛋白70(HSP70)-hom基因多态性对工人氧化应激水平影响的交互作用。方法采用判断抽样方法,以从事钒接触作业的87名工人为钒接触组,63名化工厂工人为对照组;检测血清总超氧化物歧化酶(SOD)、锰SOD、铜锌SOD、诱导型一氧化氮合酶(i NOS)的活力和MDA水平,采用限制性片段长度多态性-聚合酶链反应法检测HSP70-hom基因型分布,分析HSP70-hom基因多态性对钒接触人群氧化应激水平的综合影响。结果与对照组比较,钒接触组人群血清总SOD和锰SOD活力均下降(P<0.01),MDA水平升高(P<0.01)。HSP70-hom的T/T基因型组人群血清总SOD活力低于T/C+C/C基因型组人群(P<0.01)。单因素Logistic回归分析结果显示,HSP70-hom基因型与钒接触的交互项与血清总SOD活力和MDA高水平的发生均有关联(P<0.05)。多因素Logistic回归分析和叉生分析结果显示,HSP70-hom基因型与钒接触对血清总SOD活力偏高发生的具有相乘交互作用[比值比(OR)及其95%可信区间(CI)为6.051(1.001~36.572),P=0.05],并具有相加的交互作用,交互作用归因比及其95%CI为0.935(0.086~1.783);血清MDA水平偏高的发生仅与钒接触有关[OR(95%CI)为6.352(3.099~13.021),P<0.01],钒接触与HSP70-hom基因型对血清MDA水平偏高的发生无交互作用。未发现钒接触或HSP70-hom基因多态性对血清铜锌SOD和i NOS活力有影响。结论钒及其化合物可通过影响血清SOD和MDA而导致钒接触人群发生氧化损伤;钒接触与HSP70-hom基因多态性对作业工人血清总SOD活力偏高的发生具有交互作用。
Objective To investigate the interaction between occupational vanadium exposure and heat shock protein 70 (HSP70) -hom gene polymorphism on oxidative stress in workers. Methods Eighty-seven workers engaged in vanadium exposure were treated with vanadium and 63 workers were used as control. The serum total superoxide dismutase (SOD), manganese (SOD), copper-zinc (SOD), inducible Nitric oxide synthase (iNOS) activity and MDA levels were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR). The HSP70-hom genotype distribution was analyzed by restriction fragment length polymorphism (PCR) The combined effect of stress levels. Results Compared with the control group, the activities of serum total SOD and manganese SOD decreased (P <0.01) and the levels of MDA increased (P <0.01) in vanadium exposure group. The serum SOD activity in HSP70-hom T / T genotype group was lower than that in T / C + C / C genotype group (P <0.01). Logistic regression analysis showed that the interaction between HSP70-hom genotypes and vanadium was correlated with the total serum SOD activity and MDA level (P <0.05). Multivariate logistic regression analysis and cross-over analysis showed that the HSP70-hom genotype had a multiplicative interaction with vanadium on serum total SOD activity [odds ratio (OR) and 95% confidence interval (CI) (6.051 (1.001-36.572), P = 0.05], and had additive interactions with attribution of interaction and its 95% CI of 0.935 (0.086-1.783). High levels of serum MDA occurred only in contact with vanadium There was no interaction between vanadium exposure and HSP70-hom genotype on the high level of serum MDA with [OR (95% CI) 6.352 (3.099 ~ 13.021), P <0.01]. No vanadium exposure or HSP70-hom gene polymorphism was found to have effects on serum copper-zinc-SOD and i-NOS activity. Conclusion Vanadium and its compounds can cause oxidative damage in vanadium-exposed people by affecting serum SOD and MDA. Vanadium exposure and HSP70-hom gene polymorphism have an interaction effect on the high serum SOD activity of workers.