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本文用多种有机溶剂对紫球藻中的类脂进行提取.利用气相色谱法对提取物中AA和EPA的含量进行了分析测定,并用光谱吸收法比较了提取物中脂溶性物质一色素的含量。结果表明,氯仿-甲醇和乙醇是两种有效的提取剂,提取效果较好:紫球藻细胞中类脂含量为8%~12%,并且国培养条件不同细胞中的类脂含量也有所不同。
In this paper, we use a variety of organic solvents to extract the lipids in the P. sp. The contents of AA and EPA in the extracts were determined by gas chromatography, and the content of the lipid-soluble substances in the extracts was compared by the spectral absorption method. The results showed that chloroform-methanol and ethanol are two effective extractants, and the extraction effect is good: the lipid content in Hoechstria cells is 8%-12%, and the lipid content in different cells in different culture conditions is also different. .