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为明确4.5%丙.壳寡糖可溶性液剂对番茄灰霉病的控制效果,采用菌丝生长速率法和悬滴法测定4.5%丙.壳寡糖可溶性液剂对番茄灰霉病菌的室内毒力,并采用常规喷雾法测定该药剂对番茄灰霉病的果实组织、盆栽的影响及田间药效。室内生物测定结果表明,该药剂对番茄灰霉病菌(Botrytis cirerea)菌丝生长和孢子萌发的有效抑制中质量浓度(EC50)分别为1.56mg/L和2.60mg/L,共毒系数(CTC)分别为566.9和641.58,表现为显著增效作用。组织法试验和盆栽试验结果表明,在225mg/L剂量下,该药剂对番茄灰霉病有较好的保护作用和治疗作用,组织法对病害防效为78.06%和61.21%,盆栽试验对病害防效为51.36%和58.49%,在同样剂量下,该试剂对番茄灰霉病田间药效试验防效分别为58.47%(叶片)和64.21%(果实)。因此,4.5%丙.壳寡糖可溶性液剂对番茄灰霉病具有较好的防治效果,在农业生产上值得开发利用。
To clarify the control effect of 4.5% C. chitosan oligosaccharide soluble solution on Botrytis cinerea, indoor toxicity of Botrytis cinerea to 4.5% C was determined by mycelial growth rate and hanging drop method Force, and the use of conventional spray method to determine the effects of the agent on tomato gray mold disease of fruit tissue, potted and field efficacy. The indoor bioassay results showed that the effective inhibitory concentrations (EC50) of the agent on mycelial growth and spore germination of Botrytis cirerea were 1.56 mg / L and 2.60 mg / L, respectively. The co-toxicity coefficient (CTC) Respectively 566.9 and 641.58, showed a significant synergistic effect. Tissue culture and pot experiments showed that the agent had a good protective effect and therapeutic effect on Botrytis cinerea under a dose of 225 mg / L. The disease-control effect was 78.06% and 61.21% by tissue method, respectively. The control efficacies were 51.36% and 58.49%, respectively. At the same dose, the control efficacy of the agent on tomato gray mold was 58.47% (leaf) and 64.21% (fruit) respectively. Therefore, 4.5% C-soluble oligochitosan solution on tomato gray mold disease has good control effect, deserved to be developed and utilized in agricultural production.