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在电镜中加入由三角磁棱镜和静电反射镜所组成的电子能量分光器能分散因非弹性散射损失了能量的入射电子。利用能量弥散面上的光阑能提高生物厚切片和未经染色的薄切片的弹性明场象的分辨率和反差,也改善弹性暗场象的质量。把非弹性散射造成的能量损失量加进加速电压中,能使非弹性成象。这种“能量损失谱象”能明显改善厚切片图象质量。利用能量损失谱中的元素特性损失峰值,可获得超薄切片样品中元素分布图,并可用人工色彩对元素进行平行复制。
The addition of an electron energy beam splitter consisting of a triangular magnetic prism and an electrostatic mirror to an electron microscope disperses incident electrons that lose their energy due to inelastic scattering. The use of an aperture on the energy dispersive surface improves the resolution and contrast of the elastic bright field images of thick sections and non-dyed thin sections, and also improves the quality of elastic dark field images. The inelastic scattering caused by the energy loss added to the acceleration voltage, can make inelastic imaging. This “energy loss spectrum” can significantly improve the quality of thick slice images. Using elemental loss peaks in the energy loss spectrum, elemental profiles in ultrathin sections can be obtained and elements replicated in artificial colors.