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Aim:To examine the basic expression levels of N-methyl-D-aspartate(NMDA)receptor NR 1 and NR2B subunits in six brain regions of Sprague-Dawley(SD)ratswith different visual recognition memory.Methods:Rats were tested by a novel-object-recognition model and grouped into the high and the low visual recogni-tion memory groups.The expression levels of NR1 and NR2B subunits in thecortex,hippocampus,striatum,amygdala,diencephalon,and olfactory bulb weremeasured by semiquantitative immunoblotting.Results:The NR1 and NR2Bsubunit protein levels in the hippocampus of the high visual recognition memorygroup were 35.9%(P<0.01)and 53.4%(P<0.05)higher respectively than those inthe low group.In addition,the NR2B level in the striatum in the high visualrecognition memory group was 25.0%(P<0.05)higher than that in the low one.However,no significant difference was found in the levels of the subunits be-tween the two groups in other brain regions.Conclusion:The visual recognitionmemory in rats is related to the basic expression level of NMDA receptor NR1/NR2B subtype in the hippocampus and striatum.
Aim: To examine the basic expression levels of N-methyl-D-aspartate (NMDA) receptor NR 1 and NR2B subunits in six brain regions of Sprague-Dawley (SD) rats with different visual recognition memory. Methods: Rats were tested by a novel -object-recognition model and grouped into the high and the low visual recogni-tion memory groups. The expression levels of NR1 and NR2B subunits in the cortex, hippocampus, striatum, amygdala, diencephalon, and olfactory bulb weremeasured by semiquantitative immunoblotting. Results: The NR1 and NR2Bsubunit protein levels in the hippocampus of the high visual recognition memory group were 35.9% (P <0.01) and 53.4% (P <0.05) higher respectively than those inthe low group.In addition, the NR2B level in the striatum in the high visual prognostic memory group was 25.0% (P <0.05) higher than that in the low one. However, no significant difference was found in the levels of the subunits be-tween the two groups in other brain regions. Confluence: The visual recognition memory in rats is related to the basic expression level of NMDA receptor NR1 / NR2B subtype in the hippocampus and striatum.