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目的探索不同外水相组成对胰岛素纳米颗粒胶体系统分散性的影响,为深入开展纳米载药颗粒研究提供依据。方法采用高速高压联合均质机,经乳化-溶剂挥发法制备不同粒径的纳米载药颗粒,再通过光学显微镜、扫描电镜、激光粒径分析仪分析颗粒在胶体系统中的分散情况。结果高速均质和高速高压均质聚乙烯醇组粒径分布(d50)分别为(5·410±0·487)μm和(0·389±0·034)μm,高速均质和高速高压均质偏磷酸钠-柠檬酸盐缓冲液组粒径分布(d50)分别为(5·505±0·854)μm和(0·204±0·020)μm。联合均质结果缓冲液组粒径分布小于聚乙烯醇组,颗粒表面光滑平整;比表面积与之相反,聚乙烯醇组低于缓冲液组。结论偏磷酸钠-柠檬酸盐缓冲液作为乳化-溶剂挥发法制备胰岛素纳米载药颗粒的外水相,能够获得更好的分散体系。
Objective To explore the influence of different external aqueous phase components on the dispersibility of insulin nanoparticles colloidal system and provide the basis for further study on the drug-loaded nanoparticles. Methods High-speed and high-pressure combined homogenizer was used to prepare nanoparticles with different diameters by emulsion-solvent evaporation method. The dispersion of particles in the colloidal system was analyzed by optical microscope, scanning electron microscope and laser particle size analyzer. Results The particle size distribution (d50) of high-speed homogenization and high-speed homogenization of polyvinyl alcohol were (5.410 ± 0.487) μm and (0.389 ± 0.34) μm, respectively. The particle size distribution (d50) of the sodium metaphosphate-citrate buffer group was (5 · 505 ± 0 · 854) μm and (· · · 204 ± 0 · 020) μm, respectively. The particle size distribution of the joint homogeneous result buffer group was smaller than that of the polyvinyl alcohol group, and the surface of the particle was smooth and smooth. In contrast, the specific surface area was lower in the polyvinyl alcohol group than in the buffer group. Conclusion Sodium metaphosphate-citrate buffer can be used as emulsifying-solvent evaporation method to prepare the outer aqueous phase of insulin nanoparticles.