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目的：研究庆大霉素（ＧＭ） 致聋豚鼠耳蜗一氧化氮合酶（ＮＯＳ） 的分布及其变化，探讨一氧化氮（ＮＯ） 与ＧＭ 耳毒机制的关系。方法：ＮＡＤＰＨｄ 酶组化方法及显微图像分析技术。结果：ＧＭ 致聋后，ＮＯＳ 阳性反应物密度在血管纹、内毛细胞、外毛细胞、螺旋神经节、听神经纤维的分布均比对照组明显增高（ Ｐ＜ ０ ．００１）；上述部位的ＮＯＳ平均灰度值降低与对照组相比有显著差异（ Ｐ＜ ０ ．００１）；ＡＢＲ 听阈变化与ＮＯＳ平均密度及灰度值变化高度相关（ 上述各部位ｒ 值范围，ｒ密度听阈：０ ．９１４０～０．８６９８；ｒ灰度听阈：－０ ．７８９２ ～－ ０．９３４７；Ｐ＜ ０．０１）。结论：①ＮＯ 可能与ＧＭ的耳毒作用机制有关，是耳毒作用的重要因素之一；②ＧＭ 耳毒作用部位不仅发生在血管纹和外毛细胞，而且还涉及到内毛细胞、螺旋神经节及听神经纤维
Objective: To investigate the distribution and changes of nitric oxide synthase (NOS) in cochlea of guinea pig induced by gentamycin (GM) and to explore the relationship between nitric oxide (NO) and GM ototoxicity. Methods: NADPH d enzyme histochemistry and microscopic image analysis techniques. Results: After GM deafness, the density of NOS positive reaction was significantly higher in vascular pattern, inner hair cells, outer hair cells, spiral ganglion and auditory nerve fibers than those in control group (P <0. 001). The NOS The average gray value decreased significantly compared with the control group (P <0 .001). The change of ABR hearing threshold was highly correlated with the average density and gray value of NOS (r value range, r density hearing threshold: 0). 9140 ~ 0.8698; r gray hearing threshold: -0 .7892 ~ - 0.9347; P <0.01). Conclusions: ①NO may be related to the mechanism of ototoxicity of GM, which is one of the important factors of ototoxicity. ②GM ototoxicity not only occurs in vascular pattern and outer hair cells, but also involves the inner hair cells, spiral ganglion and Listen to nerve fibers