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目的 探讨可溶性细胞间黏附分子 - 1(s ICAM- 1)和血管内皮细胞黏附分子 - 1(s VCAM- 1)在支气管哮喘发病中的意义。方法 对支气管哮喘患者 2 3例和健康人 2 0名 (年龄均在 15～ 45岁之间 ) ,采用酶联免疫双抗体夹心法 (EL ISA)测定血清中的 s ICAM- 1和 s VCAM- 1;利用荧光酶联免疫法 (Uni- CAP系统 )分析血清中总 Ig E(t Ig E)和特异性 Ig E(s Ig E)。结果 血清 s ICAM- 1、s VCAM- 1、t Ig E水平测定哮喘组均高于对照组 (P<0 .0 1) ;血清中以户尘螨和蒿草花粉的特异性 Ig E含量增高为主 ;经多元逐步回归分析提示血清总 Ig E含量与s ICAM- 1存在线性关系 ,其复相关平方 (R- square)为 0 .5 0 2 ,标准偏回归系数为 0 .0 97,t=2 .841,P=0 .0 2 18。结论 支气管哮喘患者的 s ICAM- 1和 s VCAM- 1含量显著高于正常人 ;血清 s ICAM- 1与血清总 Ig E存在线性依存关系。
Objective To investigate the significance of soluble intercellular adhesion molecule - 1 (s ICAM - 1) and vascular endothelial cell adhesion molecule - 1 (s VCAM - 1) in the pathogenesis of bronchial asthma. Methods Twenty-three patients with bronchial asthma and 20 healthy subjects (aged 15-45 years) were enrolled in this study. Serum levels of s ICAM-1 and s VCAM-1 were measured by enzyme-linked immunosorbent assay (ELISA) 1; Total Ig E (t Ig E) and specific Ig E (s Ig E) in serum were analyzed by fluorescence enzyme-linked immunosorbent assay (Uni- CAP system). Results Serum levels of s ICAM-1, s VCAM-1 and t Ig E were higher in the asthma group than those in the control group (P <0.01). The serum IgE levels in house dust mite and wormwood pollen increased . The multiple stepwise regression analysis showed that there was a linear relationship between the total Ig E level and s ICAM-1 in serum, with a complex correlation square (R-square) of 0.550 and a standard partial regression coefficient of 0.97, t = 2 .841, P = 0 .0 2 18. Conclusion The levels of s ICAM-1 and s VCAM-1 in patients with bronchial asthma were significantly higher than those in normal controls. There was a linear relationship between serum s ICAM-1 and total serum IgE.