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目的探讨肝癌缺失基因(DLC1)与胃癌及其临床分期、分化程度等关系,DLC1mRNA表达与启动子甲基化的关系。方法用半定量逆转录聚合酶链反应方法检测34例原发性胃癌及癌旁正常组织中DLC1mRNA表达;用甲基化特异聚合酶链反应法(MSP)检测启动子甲基化。结果正常组织中DLC1mRNA均正常表达,胃癌组织DLC1mRNA低表达或表达缺失;正常组织0.62±0.11,胃癌组织0.24±0.17,差异有统计学意义(P<0.01)。DLC1mRNA表达与淋巴结转移和肿瘤分化程度有关,与性别、肿瘤大小和临床分期无关。正常组织中未发现DLC1基因启动子甲基化;胃癌组织中DLC1启动子甲基化阳性12例,甲基化率35.3%。启动子甲基化与无甲基化患者间DLC1mRNA表达差异有统计学意义(P<0.01)。结论DLC1与胃癌存在明显相关性,是重要的抑癌基因。启动子甲基化与DLC1mRNA表达抑制密切相关,可能是影响DLC1在胃癌中低表达的最主要因素,DLC1是胃癌甲基化谱中主要成员。
Objective To investigate the relationship between DLC1 gene expression and gastric cancer and its clinical stage, differentiation and so on. The relationship between DLC1 mRNA expression and promoter methylation was explored. Methods The expression of DLC1 mRNA in 34 cases of primary gastric cancer and adjacent normal tissues was detected by semiquantitative reverse transcription polymerase chain reaction (RT - PCR). Methylation - specific polymerase chain reaction (MSP) was used to detect the promoter methylation. Results The expression of DLC1mRNA in normal tissue was normal and the expression of DLC1mRNA was absent in normal gastric tissue. The normal tissue was 0.62 ± 0.11 and the gastric cancer tissue was 0.24 ± 0.17, the difference was statistically significant (P <0.01). The expression of DLC1mRNA correlated with lymph node metastasis and tumor differentiation, but not with gender, tumor size and clinical stage. DLC1 gene promoter methylation was not found in normal tissues; 12 cases of methylation of DLC1 promoter in gastric cancer tissues, the methylation rate was 35.3%. There was a significant difference in DLC1mRNA expression between promoter methylation and non-methylation (P <0.01). Conclusion DLC1 is significantly associated with gastric cancer and is an important tumor suppressor gene. Promoter methylation is closely related to the inhibition of DLC1 mRNA expression, which may be the most important factor affecting the low expression of DLC1 in gastric cancer. DLC1 is a major member of gastric cancer methylation profile.