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地黄(Rehmannia glutieosa Libosch)是一种常用中药材。在地黄产区,常有地黄黄斑病发生。叶产生近似圆形的黄色病斑,地下块茎退化,造成减产。需要探明地黄黄斑病的病原,以便有效地进行综合防治。取表现典型黄斑症状,经-20℃低温冰冻的地黄病叶100克。加入1～1.5倍体积0.2M、pH7.2磷酸缓冲液,用绞肉机绞碎,纱布过滤。将所得汁液离心20分钟(3000转/分),弃去细胞残渣。取上清液与20％氯仿混和,在分液漏斗中振荡3分钟,弃去下面氯仿层及变性蛋白层。在上层清液中,加入聚乙二醇(分
Rehmannia glutieosa Libosch is a commonly used Chinese herbal medicine. In the yellow areas, yellow patches often occur. Leaves produce a nearly circular yellow lesion, the degradation of underground tubers, resulting in reduced production. Need to prove the pathogen of yellow cambogia in order to effectively carry out comprehensive prevention and treatment. To take the typical symptoms of macular degeneration, frozen at -20 ℃ earthworm leaves 100 grams. Add 1 ~ 1.5 times the volume of 0.2M, pH7.2 phosphate buffer, minced with a meat grinder, gauze filter. The resulting juice was centrifuged for 20 minutes (3000 rpm) and the cell debris discarded. The supernatant was mixed with 20% chloroform, shaking in a separatory funnel for 3 minutes, discard the chloroform layer and denatured protein layer below. In the supernatant, add polyethylene glycol (minutes