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目的:分析中药黄连引起新生小白鼠黄疸的相关因素。方法:选择3～16天新生小白鼠42只分黄连1组、黄连2组、黄连3组、生理盐水对照组。分别腹腔注射硫酸黄连素注射液(小檗硷)0.001mg/g、0.005mg/g、0.01mg/g和0.2ml生理盐水1次。观察小鼠活动情况。3天后小白鼠尾静脉采血、离心,取血清检测总胆红素(TBiL)、结合胆红素(OBiL)、未结合胆红素(DBiL)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)。脱颈椎处死小鼠,取肝脏进行病理切片检查。结果:黄连1、2、3组小白鼠TBiL、OBiL、DBiL、ALT、AST均有明显异常改变(P<0.05);肝脏病理检查均有炎症性改变。结论:黄连可引起新生小鼠黄疸、肝脏生理功能异常。
Objective: To analyze the related factors of jaundice caused by Chinese medicine berberine. Methods: Forty-two New Zealand white mice divided into three groups were divided into two groups: Coptis 1, Coptis 2, Coptis 3, and saline control groups. Intraperitoneal injection of berberine sulfate (berberine) 0.001mg / g, 0.005mg / g, 0.01mg / g and 0.2ml normal saline once. Observed mouse activity. Three days later, the tail vein of mice was collected for blood and centrifuged, and the total bilirubin (TBiL), bound OBiL, DBiL, ALT, Aminotransferase (AST). The mice were sacrificed and the liver was taken for pathological examination. Results: The TBiL, OBiL, DBiL, ALT and AST of Coptis chinensis 1, 2 and 3 groups were all significantly changed (P <0.05). All the pathological changes of liver were changed. Conclusion: Coptis can cause neonatal mice jaundice, liver physiological function abnormalities.