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目的:研究忧遁草Clinacanthus nutans枝叶乙酸乙酯部位的化学成分。方法:采用反复硅胶柱色谱法,Sephadex LH-20柱色谱法,MCI柱色谱法等分离纯化手段相结合对忧遁草进行分离纯化,并通过波谱数据(1H-NMR,13C-NMR)鉴定其化学结构。结果:从忧遁草枝叶中分离鉴定了10个化合物,分别鉴定为吲唑(1),loliolide(2),(3S,5R,6S,7E)-5,6-环氧-3-羟基-7-大柱香波龙烯-9-酮(3),植醇(4),(-)-α-tocospirone(5),α-nigerose(6),clerspide A(7),(2R)-1-O-glyceryl-β-Dgalactoside(8),豆甾醇(9),胡萝卜苷(10)。结论:化合物2~8为首次从忧遁草枝叶中分离得到,为阐明忧遁草的药效物质基础提供了有力证据,为海南产的忧遁草资源的开发利用提供了科学依据。
Objective: To study the chemical constituents of ethyl acetate in branch and leaves of Clinacanthus nutans. METHODS: Zaodan grass was isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 column chromatography and MCI column chromatography. The compounds were identified by spectral data (1H-NMR, 13C-NMR) Chemical structure Results: Ten compounds were isolated and identified from Zygophthalmus foliaceae and identified as indazole (1), loliolide (2), (3S, 5R, 6S, 7E) (-) - α-tocospirone (5), α-nigerose (6), clerspide A (7), (2R) -1 -O-glyceryl-β-Dgalactoside (8), stigmasterol (9), daucosterol (10). CONCLUSION: Compounds 2 ~ 8 were isolated from Zhansu grass leaves for the first time, which provided powerful evidences for elucidating the material basis of Zaandan grass and provided a scientific basis for the development and utilization of Zunshi grass resources in Hainan.