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目的探索建立硬通道少创伤的自体血注射猪脑出血模型,尽量减小实验动物在实验过程中遭受的痛苦并尽可能与临床实际情况保持一致。方法采用骨穿针作为注血工具,以猪眼眶作为穿刺点定位体表标志,采用小儿生命体征监护仪在术中对猪的血压等进行监测。采用头部CT扫描和组织学方法评价血肿制作的情况并分析两者之间的联系。结果 7头小猪均成功获得了满意的额叶血肿,猪生存状态良好。影像学和组织学血肿体积相差没有统计学意义(P>0.05),两者之间有较好的相关性(r=0.931,P=0.002)。结论我们改良的猪脑出血造模方法简单、可行,造模成功率高,较好地体现了对实验动物的人性关怀,CT血肿基本可以替代组织学血肿的信息。
OBJECTIVE: To establish a mini-trauma-less pig model of intracerebral hemorrhage with minimal trauma and to minimize the suffering of experimental animals during the experiment and to keep them consistent with clinical practice. Methods The bone puncture needle was used as a blood injection tool, the pig’s orbit as a puncture point to locate the body surface marker, and the pediatric vital sign monitor to monitor the blood pressure of the pig during operation. Head CT scanning and histological evaluation of hematoma production and analysis of the relationship between the two. Results Seven piglets were successfully treated with frontal hematoma and the pigs survived well. There was no significant difference in the volume of hematoma between imaging and histology (P> 0.05), and there was a good correlation between them (r = 0.931, P = 0.002). Conclusion Our improved model of pig’s intracerebral hemorrhage is simple and feasible, with high success rate of modeling. It reflects the human care of experimental animals well. CT hematoma can basically replace the information of histological hematoma.