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为寻找与烟叶TSNA含量显著关联的标记位点,利用分布于烟草24个染色体具多态性的SSR标记和MFLP标记,分析24份烟草种质材料的遗传多样性,在此基础上对烟叶中烟草特有亚硝胺(TSNA)含量的表型变异与标记多态性进行关联分析。结果显示,33对MFLP引物和28对SSR引物在24份烟草材料中共发现188个多态位点;群体结构分析将24份烟草材料分为3个亚群,且亚群划分与烟草类型(烤烟、晾晒烟和白肋烟)基本一致;关联分析发现1个SSR位点和6个MFLP位点至少与1种TSNA含量的相关性在0.01水平上显著,其中标记MFLP26与烤后烟叶中NNN含量的相关性在0.001水平上显著,表型变异解释率最高(R2=0.5831)。这些显著关联的分子标记可为筛选低TSNA含量的烟草材料提供参考。
In order to find the marker sites significantly associated with the content of TSNA in tobacco leaves, the genetic diversity of 24 tobacco germplasms was analyzed using SSR markers and MFLP markers distributed in tobacco 24 chromosomes. Based on this, Tobacco-specific nitrosamines (TSNA) phenotypic variation and marker polymorphism were analyzed. A total of 188 polymorphic sites were detected in 33 pairs of MFLP primers and 28 pairs of SSR primers in 24 tobacco cultivars. The population structure analysis divided 24 tobacco materials into 3 subgroups, , And dried tobacco and burley tobacco). Correlation analysis showed that the correlation between at least one TSNA content of one SSR locus and six MFLP loci was significant at 0.01 level. The correlation between MFLP26 marker and NNN content in roasted tobacco leaves The correlation was significant at the level of 0.001, the highest rate of interpretation of phenotypic variation (R2 = 0.5831). These significantly associated molecular markers can provide a reference for screening tobacco material with low TSNA content.