心肌疾病可引起心肌细胞肥大,功能性心肌细胞数量减少,成为不可逆的改变,而导致心力衰竭。心肌疾病的发病机制极为复杂,近年来研究发现,心力衰竭时心肌细胞的钾、钠、钙等离子通道的功能表现出特征性的变化,应用膜片箝技术探寻其心肌细胞膜的离子通道功能及其特点,有望进一步深入对心肌疾病的发病机制及其治疗方法的研究,已成为当今的一个热点。现就膜片箝技术的概念、产生、原理、方法以及在国内外心肌疾病发病机制研究中的应用现状及发展趋势作一综述。 Cardiac disease can cause hypertrophy of cardiomyocytes, decreased number of functional cardiomyocytes, irreversible changes that lead to heart failure. The pathogenesis of myocardial disease is extremely complicated. In recent years, it has been found that the function of potassium, sodium and calcium ion channels of cardiomyocytes in heart failure show the characteristic changes. Patch-clamp technique is used to explore the function of ion channels of myocardial cell membrane and its Characteristics, is expected to further study the pathogenesis of myocardial disease and treatment methods, has become a hot spot today. Now the concept of patch clamp technology, production, principles, methods and application in the study of the pathogenesis of myocardial disease at home and abroad, the status quo and development trends are reviewed.