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系统性红斑狼疮 (SLE )的病因和诱导抗核抗体 (ANA )生成的激发原迄今不明。本实验试图用ConA活化淋巴细胞的染色质免疫同系BALB/c小鼠 ,寻找诱导ANA生成的真正免疫原 ,阐明SLE发生的激发原是自身活化细胞的核成分 ,而且证明它所诱导的ANA具有致病性。从ConA活化的脾细胞中提取染色质 ,然后免疫同系BALB/c小鼠 ,用ELISA方法测定IgG类抗双链DNA (dsDNA )、抗组蛋白抗体 ,用免疫荧光法检测抗核抗体核型和免疫复合物沉积 ,用免疫印迹法测定抗核抗体谱 ,在光镜下检测肾损伤及电镜下检测肾小球沉积物 ,用考马斯亮蓝法检测尿蛋白含量。结果显示 ,活性染色质能诱导IgG类抗dsDNA、抗组蛋白等多种抗核抗体生成 ,且肾小球有显著免疫复合物沉积和蛋白尿形成。该实验表明 ,活化淋巴细胞的染色质是诱导SLE发生的真正自身免疫原。
The etiology of systemic lupus erythematosus (SLE) and the provocations that induce the generation of antinuclear antibodies (ANAs) have so far been unknown. In this study, we attempted to immunize the BALB / c mice with ConA-activated lymphocytes to find the true immunogen that induces the formation of ANA, elucidating the nuclear component of SLE that is activated by autologous cells and proving that the ANA it induces Pathogenicity. Chromatin was extracted from ConA-stimulated splenocytes, then the BALB / c mice were immunized, and IgG anti-double stranded DNA (dsDNA) and anti-histone antibodies were detected by ELISA. The karyotype of anti-nuclear antibodies Immunocomplex deposition, anti-nuclear antibody spectrum by immunoblotting, renal damage detected by light microscopy and electron microscopic examination of glomerular deposits, urinary protein content by Coomassie brilliant blue method. The results showed that active chromatin can induce IgG anti-dsDNA, anti-histone and many other anti-nuclear antibody production, and glomerular significant immune complex deposition and proteinuria. This experiment shows that the chromatin of activated lymphocytes is a true auto-immunogen that induces the development of SLE.