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以骆驼刺(Alhagi pseudalhagi Desv.)叶片为试验材料,研究了基本培养基、不同叶位、放置方式及植物生长调节剂配比等对不定芽再生的影响。结果表明:骆驼刺叶片再生的最佳取材位置是试管苗中部;不同放置方式对不定芽的再生无明显影响;叶片不定芽再生的最佳培养基为:MS+6-BA 0.5 mg.L-1+IBA 0.1 mg.L-1,其不定芽分化率为100%,分化系数达11.28。低浓度IAA有利于叶片再生茎段的生根,在1/2MS+IAA 0.15 mg.L-1的培养基上生根率为100%,平均根数达10.2条。
The leaf of Alhagi pseudalhagi Desv. Was used as experimental material to study the effects of basic medium, different leaf positions, placement methods and plant growth regulators on the regeneration of adventitious buds. The results showed that the best medium for regeneration of camel’s leaves was the middle part of test-tube plantlets. Different placement methods had no significant effect on the regeneration of adventitious buds. The best medium for adventitious bud regeneration was MS + 6-BA 0.5 mg.L- 1 + IBA 0.1 mg.L-1, the adventitious bud differentiation rate was 100% and the differentiation coefficient was 11.28. Low concentration of IAA was beneficial to the rooting of the regenerated stem segments. The rooting rate was 100% with the average number of rooting of 10.2 on 1/2 MS + IAA 0.15 mg.L-1 medium.