目的比较由HeLa细胞及Ehrlich瘤细胞分别提取RA33/36抗原在类风湿性关节炎(rheumatoidarthritis,RA)诊断中的敏感性和特异性。方法RA33/36抗原由HeLa细胞及Ehrlich瘤细胞提取,抗RA33/36用免疫印迹法检测。研究对象分RA患者、非RA患者及正常对照。结果HeLa细胞抗原和Ehrlich瘤细胞抗原两者检测抗RA33/36对RA诊断的特异性为85.7%和87.5%,敏感性为28.2%和26.9%。抗RA33和抗RA36对RA均有诊断价值。结论HeLa细胞及Ehrlich瘤细胞均可用于提取RA33/36抗原。 Objective To compare the sensitivity and specificity of RA33 / 36 antigen extracted from HeLa cells and Ehrlich tumor cells in the diagnosis of rheumatoid arthritis (RA). Methods RA33 / 36 antigen was extracted from HeLa cells and Ehrlich tumor cells, and anti-RA33 / 36 was detected by Western blotting. Subjects were divided into RA patients, non-RA patients and normal controls. Results The specificity of both anti-RA33 / 36 for the diagnosis of RA was 85.7% and 87.5% for both HeLa cell antigen and Ehrlich tumor cell antigen with a sensitivity of 28.2% and 26.9%, respectively. Both RA33 and anti-RA36 have diagnostic value for RA. Conclusion HeLa cells and Ehrlich tumor cells can be used to extract RA33 / 36 antigen.