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为了研究生活饮用水中低剂量污染物质对人体健康的影响,应用淡水发光细菌青海弧菌Q67检测了用生活饮用水卫生标准(GB5749—2006)限制检出的14种物质配制的混合液在不同pH值时的毒性及其不同稀释度对发光细菌发光抑制的动力学曲线。同时,为了探寻多元混合物的联合毒性对发光细菌作用的机制,分别测定了14种物质中的氯化铜、氯化锌和重铬酸钾对发光细菌的单一毒性的EC_(50),以及等浓度配比和等毒性配比的二元混合体系的联合毒性,采用相加指数法对其联合毒性效应进行了评价,同时采用统计学方法二次2因子回归通用旋转组合实验设计研究了二元混合体系的联合毒性作用。结果表明,14种限制检出物质的混合液对发光细菌有明显的抑制作用;3种重金属化合物的等浓度比和等毒性比二元混合体系的联合毒性作用结果一致,氯化铜与重铬酸钾呈拮抗作用,氯化铜与氯化锌、氯化锌与重铬酸钾均为协同作用;而采用统计学方法在本文条件下可得出:3种二元混合物的毒性效应,其相互影响因它们浓度而异。研究表明14种限制检出物质,尽管它们的浓度均符合国标有关限制浓度值,但他们共存时的联合毒性十分显著,可能对生物和人体有害。
In order to study the impact of low dose of pollutants in drinking water on human health, freshwater luminescent bacteria Qinghai Vibrio Q67 was used to test the effect of 14 kinds of substances which were limited by the standard of sanitary drinking water (GB5749-2006) Toxicity at pH and Kinetics of Inhibition of Luminescence of Luminescent Bacteria by Different Dilutions. At the same time, in order to explore the mechanism of combined toxicity of multiple mixtures on luminescent bacteria, the EC50 of single toxicity of copper chloride, zinc chloride and potassium dichromate to luminescent bacteria in 14 substances were determined, Concentration ratio and the toxicity ratio of the binary mixture system of joint toxicity, the use of additive index method to evaluate its joint toxicity, and statistical methods using the second two-factor regression universal rotation combination experimental design of the binary Combined toxicity of mixed system. The results showed that the mixture of 14 kinds of substances with limited detection obviously inhibited the luminescent bacteria. The equal concentration ratio and iso-toxicity of the three heavy metal compounds were more consistent than those of the binary mixed system. Copper chloride and heavy chromium Potassium and potassium antagonists, copper chloride and zinc chloride, zinc chloride and potassium dichromate are Synergy; and statistical methods in this paper can be drawn under the conditions: the toxicity of three binary mixtures, the Interactions vary depending on their concentration. Studies have shown that 14 kinds of restricted substances, although their concentrations are in line with the national standard concentration limit, but when they coexist joint toxicity is very significant, may be harmful to humans and humans.