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目的研究临床分离的多重耐药大肠杆菌耐消毒剂基因携带状况及其对消毒剂抗性水平。方法采用聚合酶链反应(PCR)法和体外抗菌试验方法进行实验室检测。结果在检测的8株临床分离的大肠杆菌中,检出3株携带qacE△1基因,检出率37.5%。含氯消毒剂对3株qacE△1基因阳性大肠杆菌的MIC值均高于标准菌株。碘伏消毒剂和戊二醛消毒剂只对1株qacE△1基因阳性大肠杆菌的MIC值高于标准菌株,其他均与标准株相同。结论临床分离的多重耐药大肠杆菌qacE△1基因阳性率较高,携带qacE△1基因阳性的大肠杆菌对含氯消毒剂有产生抗性的倾向。
Objective To study the status of multidrug-resistant Escherichia coli resistance to disinfectant and its resistance to disinfectants. Methods Polymerase chain reaction (PCR) and in vitro antibacterial test methods were used for laboratory testing. Results Among the 8 isolates tested, qacE △ 1 gene was detected in 3 isolates and the detection rate was 37.5%. The MICs of three chlorine-containing disinfectants against qacE △ 1 -positive Escherichia coli were higher than those of the standard strains. Iodoviral disinfectant and glutaraldehyde disinfectant only one strains of qacE △ 1 positive Escherichia coli MIC value higher than the standard strains, the other are the same as the standard strains. Conclusions The positive rate of qacE △ 1 gene of multidrug-resistant Escherichia coli isolated clinically is high, and Escherichia coli carrying qacE △ 1 gene is resistant to chlorine disinfectant.