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使冷的鸡蛋蛋黄与整个鸡蛋分开,采用水洗办法尽可能地洗去白蛋白。一次以提取3—5个蛋黄为好。具体做法是:把蛋黄去膜打碎,然后慢慢倒入冷至-20℃的异丙醇(每个蛋黄需100毫升)中,用混合振荡器充分振荡混合一会儿,静量3—5分钟,使蛋白沉淀下来,倾去上清液。为了完全去除类脂化合物,将沉淀物与同上数量的预冷异丙醇充分振荡混合两分钟,待沉淀后倾出上清液,其沉淀物用预冷丙酮再洗涤两次,最后将沉淀收集在一个瓷漏斗(用602h 滤纸)中
Make the cold egg yolk separate from the whole egg and wash it with albumin as soon as possible. Take 3-5 egg yolks at once as well. The specific approach is: the egg yolk to the membrane broken, and then slowly poured into cold to -20 ℃ isopropanol (each egg yolk required 100 ml), mixed with a shaker well mixed for a while, static 3-5 minutes , Allowing the protein to settle down, decanting the supernatant. In order to completely remove lipid compounds, the precipitate and the same number of precooling isopropanol was mixed thoroughly for two minutes. After the precipitate was precipitated, the supernatant was decanted, the precipitate was washed twice with pre-cooled acetone, and finally the precipitate was collected In a porcelain funnel (with 602h filter paper)