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目的:建立一种快速有效的大鼠腹腔异位心脏移植模型。方法:采用SD大鼠作为受体,Wistar大鼠作为供体,行同种异位腹腔心脏移植,术后给以CsA5 mg/kg/d灌服,心脏移植手术方法采用改良的Ono术式,观察改良的腹腔异位心脏移植各步骤所需时间、术后成功率及主要并发症发生率。结果:共建立40只大鼠异位心脏移植模型,手术成功率92.5%。动脉吻合时间12.5±2.3min,静脉吻合时间12.3±1.5 min,供心缺血时间37±3.5 min,受体血管阻断时间34.2±2.6 min,总手术时间90.2±4.8 min,出现的主要并发症为出血和供心复跳失败(各占5%、2.5%)。结论:改进的大鼠腹部异位心脏移植技术是一种简便、快速、有效、成功率高的模型制作方法。
Objective: To establish a rapid and effective rat heterotopic abdominal heart transplantation model. Methods: Sprague - Dawley rats were used as recipients and Wistar rats as donors. Allografts were transplanted into the abdominal cavity with CsA 5 mg / kg / d after operation. The modified heart - Observe the time required for each step of modified celiac heterotopic heart transplantation, the success rate after operation and the main complication rate. Results: A total of 40 rat heterotopic heart transplantation models were established. The successful rate was 92.5%. Arterial anastomosis time 12.5 ± 2.3min, venous anastomosis time 12.3 ± 1.5min, donor ischemia time 37 ± 3.5min, receptor vascular occlusion time 34.2 ± 2.6min, total operation time 90.2 ± 4.8min, the main complications Hemorrhage and heart failure (5% each, 2.5%). Conclusion: The improved abdominal heterotopic heart transplantation in rats is a simple, rapid, effective and successful model making method.