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研究旨在明确采自北京平谷圣泉庵橡树根际土壤中昆虫病原线虫(Steinernema longicaudum BPS)的生物学特性,进而明确开发应用的可能性。结果显示:在剂量10和20IJs/幼虫下,BPS对寄主幼虫的侵染效果相近,大蜡螟的校正死亡率分别为40.0%和36.7%,黄粉甲的分别为50.0%、53.3%;在剂量40、80、160IJs/幼虫下,BPS的侵染力随剂量的增高而增大,大蜡螟和黄粉甲的校正死亡率分别为73.3%、86.7%、90.0%和70.0%、80.0%、90.0%;在剂量320和640IJs/幼虫下的侵染效果(校正死亡率100%)相近。BPS经40℃水浴处理1h校正死亡率达98%,处理3h,线虫100%死亡;经37℃、36℃和34℃水浴处理10h,校正死亡率分别为92.4%、55.4%和45.7%,处理20h,线虫100%死亡。该线虫在30℃条件下的水平运动能力比在18℃和25℃的强,有34.6%的线虫用24h的时间运动到3.6cm处。研究结果表明BPS具有较强的侵染力、耐热性和水平扩散能力,据此初步分析BPS具有被开发应用潜能。
The aim of this study was to determine the biological characteristics of Steinernema longicaudum BPS collected from oak rhizosphere soil of Shengquan mound in Pinggu, Beijing, and to clarify the possibility of development and application. The results showed that under the conditions of 10 and 20IJs / larvae, the infection rates of BPS to host larvae were similar, and the corrected mortality rates of larvae were 40.0% and 36.7%, respectively, while the rates of yellow powder A were 50.0% and 53.3% Under 40, 80 and 160 IJs / larvae, the infectivity of BPS increased with the increase of dose, and the corrected mortalities of Baphicolicotis and Diplodocus were respectively 73.3%, 86.7%, 90.0% and 70.0%, 80.0% and 90.0 %; Infections at 320 and 640 IJs / larvae (corrected mortality 100%) were similar. The corrected mortality of BPS treated by water bath at 40 ℃ for 1 hour was 98%, treated for 3 hours, and the nematodes were 100% dead. The corrected mortalities were 92.4%, 55.4% and 45.7% respectively at 37 ℃, 36 ℃ and 34 ℃ for 10h 20h, 100% nematode death. The nematode had a higher level of exercise capacity at 30 ° C than at 18 ° C and 25 ° C, and 34.6% of nematodes moved to 3.6 cm with a time of 24 hours. The results show that BPS has strong ability of infectivity, heat resistance and horizontal diffusion. Based on this, BPS has potential of being developed and applied.