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研究了聚乙二醇改性碳纳米管(PEG-CNTs)对人胚肾细胞(293T)及人肝癌细胞(HepG2)的体外细胞毒性.在染毒24、48和72 h后,用水溶性四氮唑法检测不同浓度PEG-CNTs对细胞活性的影响;采用碘化丙啶染色研究不同浓度的PEG-CNTs在对HepG2细胞染毒24 h后死亡细胞染色情况,并用流式细胞术定量测定细胞死亡率.结果表明:水溶性较好的PEG-CNTs作用于293T细胞与HepG2细胞,其毒性存在浓度依赖关系,毒性会随着浓度的升高而增大;根据ISO2109932-5细胞毒性标准,浓度≤100 μg/mL时为毒性Ⅰ级,认为PEG-CNTs没有毒性;当浓度为200 μg/mL时,毒性变为Ⅱ级,为轻微毒性,说明低剂量的PEG-CNTs对细胞未显示毒性作用;在研究的时间范围内未发现随时间延长而毒性等级的变化.“,”The cytotoxicity of polyethylene glycol-modified carbon nanotubes (PEG-CNTs) on human embryonic kidney cells (293T) and hepatoma cells (HepG2) was studied in vitro.The effect of the concentration of PEG-CNTs on the cell activity and survival rate was detected by a colorimetric assay method after exposure for 24,48 and 72 h.The dead HepG2 cells obtained by incubation in solutions with different concentrations of PEG-CNTs for 24 h can be stained by a dye,propidium iodide,and the cell mortality was determined by flow cytometry.Results show that the toxicity of PEG-CNTs with a good water solubility towards 293T and HepG2 cells increases with increasing PEG-CNT concentration.When the concentration of the PEG-CNTs is less than 100 μg/mL,the toxicity of the PEG-CNTs is Grade Ⅰ (non-toxic) and when it is greater than 100 μg/mL but less than 200 μg/mL it is Grade Ⅱ (mildly toxic),according to the ISO2109932-5 cytotoxicity standard.The toxicity grade does not change with increased time.