论文部分内容阅读
目的:研究系统性红斑狼疮(SLE)患者外周血中甘露糖结合凝集素(MBL)水平与SLE的临床表现、经典的血清学指标及疾病活动性指数的相关性。方法:应用固相酶联免疫吸附(ELISA)法检测54例(其中活动期39例、缓解期15例)SLE患者和45名健康志愿者外周血MBL水平,同时检测SLE患者外周血抗dsDNA抗体、C3补体、白细胞计数及循环免疫复合物(CICs)水平,并记录SLE患者主要临床症状(肾脏系统受累、神经系统受累、严重感染等),疾病活动度用SLEDAI记分。结果:系统性红斑狼疮患者外周血MBL水平显著低于健康志愿者(P<0.01);特别是活动期SLE患者血清MBL水平明显降低,与缓解组比较差别具有统计学意义(P<0.01);SLE患者血清MBL水平与SLEDAI、抗dsDNA抗体、CICs水平呈负相关,与白细胞计数及C3补体水平呈正相关;并发严重感染的SLE患者组血清MBL水平显著低于无受累组(P<0.01)。结论:SLE患者血清MBL水平明显降低,血清MBL水平与SLEDAI、抗dsDNA抗体、C3水平、CIC水平、白细胞水平之间明显相关,血清MBL水平较低的患者更易发生比较严重的感染,血清MBL水平可能是SLE潜在生物学标记。
Objective: To study the correlation between the serum levels of mannose-binding lectin (MBL) and the clinical manifestations, classical serological markers and disease activity index in patients with systemic lupus erythematosus (SLE). Methods: Peripheral blood MBL levels were measured in 54 patients (39 active and 15 remission) and 45 healthy volunteers by solid-phase enzyme-linked immunosorbent assay (ELISA). The levels of anti-dsDNA antibody , C3 complement, white blood cell count and circulating immune complexes (CICs). The main clinical symptoms (renal involvement, nervous system involvement, severe infection, etc.) of SLE patients were recorded. The disease activity was scored with SLEDAI. Results: MBL levels in peripheral blood of patients with systemic lupus erythematosus were significantly lower than those of healthy volunteers (P <0.01). The levels of serum MBL in active SLE patients were significantly lower than those in healthy volunteers (P <0.01). Serum MBL levels in SLE patients were negatively correlated with SLEDAI, anti-dsDNA antibody and CICs levels, and positively correlated with leukocyte counts and C3 complement levels. Serum MBL levels were significantly lower in SLE patients with severe infection than those without SLE (P <0.01). CONCLUSIONS: Serum MBL levels are significantly decreased in patients with SLE. Serum MBL level is significantly associated with SLEDAI, anti-dsDNA antibody, C3 level, CIC level and leukocyte level. Serum MBL levels are more likely to occur in patients with lower serum MBL levels It may be a potential biological marker for SLE.